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Bacteriology

Modification Of Carry-Blair Transport Media For Storage Salmonella typhi

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Pages 72 - 73

Abstract

The aim of this study was to determine transport media modification as alternative media to replace Carry Blair. One type of transport media that often use to carry faeces specimens suspected to contain Salmonella typhi is Carry-Blair media. Studies have been conducted experimentally by storing Salmonella typhi on alternative transport media with Peptone composition, disodium Phosphate, Sodium chloride, Calcium chloride, which is made using a semi-solid and Carry-Blair as a control. Three variety of storage was done (0 hour,6 hours,9 hours) at a temperature 4â°-8â°C and then Salmonella typhi was inoculated in Salmonella Shigella Agar using spread plate technique incubated during 24 hours at 37â°C, counted the number of colonies by the plate count method using the colony counter. The results of ANOVA could be concluded that modification media could be use as alternative media replace Carry-Blair at 6 hours. Based on regression correlation test was assumed that the Salmonella typhi bacteria still life at less than 11 hours 54 minutes.

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References

[1] Wheeler and Volk, Dasar - Dasar Mikrobiologi. Jakarta: Erlangga, 1993.
[2] G. Tefera and J. Smola, “Modification of Cary-Blair Transport Medium for Pasteurella multocida and Mannheimia haemolytica,” 2002. .
[3] U. Suryawiria, Pengantar Mikrologi Umum. Bandung: Angkasa, 1995.
[4] Pelzcar and Chan, Dasar - Dasar Mikrobiologi. Jakarta: UI-Press, 1986.
[5] L. Waluyo, Mikrobiologi Umum. Malang: Universitas Muhamadiah Malang Press, 2005.
[6] I. Kurniati, Penuntun dan Jurnal Praktikum Bakteriologi (Cetakan 1). Bandung: Akademi Analis Kesehatan Bakti Asih, 2009.
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How to Cite This

Supriatin, Y., & Rahayyu, M. (2016). Modification Of Carry-Blair Transport Media For Storage Salmonella typhi. Jurnal Teknologi Laboratorium, 5(2), 72–73. Retrieved from https://www.teknolabjournal.com/index.php/Jtl/article/view/83

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