1370
Abstract Views
1714
PDF Download
Bacteriology

Identifikasi Gen Ctx-M Pada Esherichia Coli Penghasil Extended Spectrum Beta-Lactamases (Esbls) Di RSUD Dr. Soetomo surabaya

Pages 56 - 60

Abstract

Escherichia coli producing Extended-Spectrum Beta-Lactamases (ESBLs) responsible for the high number of disease and death from nosocomial infections because of an enzyme encoded by genes CTX-M. This aim of the research to identify isolates E.coli of urine patients, which is a collection of Clinical Microbiology RSUD Dr. Soetomo Surabaya on the January-February 2014. The kind of the research use is observational descriptive with molecule approachment. The sample used in clinical isolate of E.coli producing ESBLs accumulated during January-February 2014 collection Clinical Laboratory RSUD Dr. Soetomo Surabaya that is thirty isolate. The methods used for the detection of genotypic by PCR thus electrophoresis and visualized on agarose gel 1.5%. The results show that twenty-seven isolate (90%) positive containing a gen CTX-M with the highest number found on the Interna Departement. Detection Bacteria producing ESBLs in genotype important that therapy an antibiotic the patients are given more effective and efficient.

There is no Figure or data content available for this article

References

[1] K. Kuntaman, S. Santoso, H. Wahjono, N. Mertaningsih, E. Lestari, H. Farida, R. Hapsari, S. Firmanti, A. Noorhamdani, D. Santosaningsih, P. Purwono and D. Kusumaningrum, "The sensitivity pattern of extended spectrum beta lactamase producing bacteria against six antibiotics that routinely used in clinical setting," Journal Indonesian Medical Association, pp. 482-486, 2011.
[2] J. Sharma, M. Sharma and P. Ray, "Detection of TEM and SHV genes in Escherichia coli and Klebsiella pneuomonia Isolates in a tertiary care hospital from India," Indian Journal of Medical Research, no. 132, pp. 332-336, 2010.
[3] B. E. Bali, L. Acik and N. Sultan, "Phenotypic and Molecular characterization of SHV, TEM, CTX-M and exteded spectrum beta lactamases produced by Esherichia coli, Acinetobacter baumanii, and Klebsiella isolates in Turkish Hospital," African Journal of Microbiology Research, vol. 8, no. 4, pp. 650-654, 2010.
[4] T. Sana, K. Rami, B. Racha, D. Fouad, A. Marcel, M. Hasan, H. Sani and H. Monzer, "Detection of genes TEM, OXA, SHV, and CTX-M in 73 clinical isolates of Escherichia coli producers of extended spectrum beta lactamases and determination of thieir suscpectibility to antibiotics," iMedPub Journals, vol. 1, no. 1, pp. 1-5, 2011.
[5] N. Jan, U. Sudhir and K. Archana, "Plasmid profile analysis of multidrug resistance E.coli isolated from UTI patients of Nagpur City, India," Romanian Biotechnological Lettes, vol. 5, no. 14, pp. 4635-4640, 2009.
[6] C. Wollheim, I. Guerra, V. Conte, S. Hoffman, F. Schreiner, A. Delamare, A. Barth, S. Echeverrigaray and S. Costa, "2011," Braz J. Infect Dis, vol. 2, no. 15, pp. 138-143, 2011.
[7] P. Kiratisin, A. Apisarnthanarak, C. Laesripa and P. Saifon, "Molecular characterization and epidemiology of extended spectrum beta lactamases producing Escherichia coli and Klebsiela pneuomoniae isolates carrying health care-assosiated infection in Thailand," Antimicrobial Agents Chemotheraphy, no. 52, pp. 2818-2824, 2011.
[8] J. Kim, Y. Lim, Y. Jeong and Y. Seol, "Occurance of CTX-M-3, CTX-M-15, CTX-M-14 and CTX-M-9 Extended Spectrum Beta Lactamases in Enterobacteriaceae Clinical Isolates in Korea," Antimicrob. Agents Chemother, vol. 4, no. 49, p. 1572, 2005.
[9] M. Edelstein, M. Pimkin, I. Palagin, I. Edelstein and L. Stratchounski, "Prevalence and molecular epidemiology of CTX-M Extended Spectrum Beta Lactamases Producing Escherichia coli and Klebsiella pneumoniae," Antimicrobioal Agents and Chemoteraphy, vol. 12, no. 47, pp. 3724-3723, 2003.
[10] J. Severin, N. Mertaningsih, K. Kuntaman, E. Lestari, M. Purwanta, N. Toom, D. Duerink, U. Hadi, A. Belkum, H. Verbrugh and W. Goessens, "Molecular characterization of extended spectrum beta lactamases in clinical Escherchia coli and Klebsiella pneumoniae isolates from Surabaya," Journal Antimicrobial Chemotheraphy, no. 65, pp. 465-469, 2010.
[11] A. Sorlozano, J. Guteirrez, J. Luna, J. Oteo, J. Liebena, M. Soto and G. Piedrola, "High presence of extended specgtrum beta lactamases and resistance to quinolones in clinical isolates of Escherichia coli," Microbiology Research, no. 162, pp. 347-354, 2007.
[12] C. Ferreira, W. Ferreira, N. Almeida, F. Gomes, Navacea and M. Barbosa, "Extended Spectrum Beta Lactamase Producing Bacteria Isolated from Hematologic Patients in Manaus, State of Amazonas, Brazil," Brazilian Journal of Microbiology, pp. 1076-1084, 2011.
[13] S. T. Pratiwi, Mikrobiologi Farmasi, Jakarta: Erlangga, 2008.
[14] M. Adelyap, S. Harbart, N. Vernaz, M. Kearney, M. Scott, F. Elhajji, A. Aldiab and J. Mc Elnay, "The impact of antibiotic use on the incidence and resitance pattern of extended spectrum beta lactamase-producing bacteria in primary and secondary healthcare settings," Bristish Journal of Clinical Pharmacology, pp. 1365-2125, 2011.
[15] P. Rao, CTX-M Beta Lactamases, Davangere: Department of Microbiology, 2012.
There is no Supplemental content for this article.

How to Cite This

Prasetya, Y. A. (2017). Identifikasi Gen Ctx-M Pada Esherichia Coli Penghasil Extended Spectrum Beta-Lactamases (Esbls) Di RSUD Dr. Soetomo surabaya. Jurnal Teknologi Laboratorium, 6(2), 56–60. https://doi.org/10.29238/teknolabjournal.v6i2.92

Article Metrics

Download Statistics

Downloads

Download data is not yet available.

Other Statistics

Verify authenticity via CrossMark

Copyright and Permissions

Publishing your paper with Jurnal Teknologi Laboratorium (JTL) means that the author or authors retain the copyright in the paper. JTL granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by JTL in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.

JTL journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially.

JTL journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:

  • BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • SA:  If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

Data Availability