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Biotechnology

DNA degradation in diabetes mellitus patients and its impact on DNA quality for forensic analysis

Egita Windrianatama Puspa Universitas Muhammadiyah Pringsewu, Indonesia Correspondence egita.windrianatama.puspa-2020@pasca.unair.ac.id
ORCID : https://orcid.org/0000-0002-1176-9574
,
Gustiadi Saputra Politeknik Kesehatan Kesuma Bangsa, Indonesia Correspondence gustiadisaputra51@gmail.com
,
Nuria Muliani Universitas Muhammadiyah Pringsewu, Indonesia Correspondence nuriamuliani@umpri.ac.id
,
Susi Susanti Universitas Muhammadiyah Pringsewu, Indonesia Correspondence susi.2023206205016@student.umpri.ac.id
,
Santika Dwi Lasmana Universitas Muhammadiyah Pringsewu, Indonesia Correspondence santika.2024206205004@student.umpri.ac.id
Pages 118-128
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Abstract

This study investigates the effect of Diabetes Mellitus (DM) on DNA degradation and its quality in forensic analysis. DNA, as a key element in forensic investigations, is susceptible to degradation due to the altered physiological state and chronic hyperglycemia prevalent in DM patients. Blood samples from DM patients with fasting blood glucose (FBG) levels greater than 126 mg/dL and HbA1c ≥7% were analyzed. The results showed a significant decrease in DNA concentration, with the control group showing 1353.2 ng/µL, and reductions observed on day 7 (989.9 ng/µL), day 14 (750.4 ng/µL), and day 20 (354.2 ng/µL). DNA purity remained relatively stable, with a slight reduction from 1.83 on day 7 to 1.74 on day 20. Statistical analysis using ANOVA indicated significant differences between time points for both DNA concentration (p < 0.05) and purity (p < 0.05). Games-Howell post hoc tests confirmed a significant decrease in DNA concentration over time (p < 0.05). The significant decrease in DNA concentration reflects DNA degradation, which worsens over time, while DNA purity remains within an acceptable range. The findings suggest that DNA samples derived from DM patients may exhibit a higher susceptibility to progressive degradation, potentially impacting the reliability of subsequent forensic analyses. This study emphasizes the need for advanced forensic techniques capable of handling degraded DNA samples, enhancing the sensitivity of forensic DNA analysis and ensuring reliable forensic identification.

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How to Cite This

Puspa, E. W., Saputra, G., Muliani, N., Susanti, S., & Dwi Lasmana, S. (2026). DNA degradation in diabetes mellitus patients and its impact on DNA quality for forensic analysis. Jurnal Teknologi Laboratorium, 15(2), 118–128. https://doi.org/10.29238/teknolabjournal.v15i2.717

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