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Biotechnology

Evaluation of tris–EDTA pre-hydrated non-FTA dried blood spot as a low-cost medium for room-temperature DNA sample transport

Putri Dwi Zakirah Febriyani Department of Medical Laboratory Technology, Poltekkes Kemenkes Palembang, Indonesia, Indonesia Correspondence putridwizakirahfebriyani24@gmail.com
,
Anton Syailendra Department of Medical Laboratory Technology, Poltekkes Kemenkes Palembang, Indonesia, Indonesia Correspondence antonsyailendra@gmail.com
,
Hamril Dani Department of Medical Laboratory Technology, Poltekkes Kemenkes Palembang, Indonesia, Indonesia Correspondence hamrildani@gmail.com
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Ocktariyana Department of Medical Laboratory Technology, Poltekkes Kemenkes Palembang, Indonesia, Indonesia Correspondence ocktariyana@gmail.com
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Aqda Rizkia Pasha Department of Medical Laboratory Technology, Poltekkes Kemenkes Palembang, Indonesia, Indonesia Correspondence aqda.rizkia@gmail.com
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Shungai Mutiara Aini Department of Medical Laboratory Technology, Poltekkes Kemenkes Palembang, Indonesia, Indonesia Correspondence shungaimutiara@gmail.com
Pages 48-58
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Abstract

Dried Blood Spot (DBS) is a practical method for collecting DNA samples; however, DNA stability on non-FTA filter paper such as Whatman No. 42 remains limited. Pre-hydration of the DBS matrix with Tris–EDTA (TE) buffer has the potential to enhance DNA stability during room-temperature storage. This study aimed to evaluate the effectiveness of TE-hydrated DBS in maintaining DNA stability for short-term storage and transport. A paired quasi-experimental design was applied in which each subject provided two DBS samples: one on TE-hydrated paper and one on unmodified paper. Twenty subjects were recruited using convenience sampling, generating 40 DBS samples for analysis. TE hydration significantly increased DNA concentration compared with unmodified DBS (p < 0.01) and increased the proportion of samples with acceptable purity (A260/A280 = 1.8–2.0) (p = 0.031). Hydration with TE buffer was therefore effective in improving DNA yield, while its effect on DNA purity remained sensitive to technical variability and outlier influence. These findings indicate that TE-hydrated DBS has potential as an alternative medium for short-term DNA transport at room temperature. Further validation involving extended storage duration and downstream molecular testing is required.

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How to Cite This

Febriyani, P. D. Z., Syailendra, A., Dani, H., Ocktariyana, Pasha, A. R., & Aini, S. M. (2026). Evaluation of tris–EDTA pre-hydrated non-FTA dried blood spot as a low-cost medium for room-temperature DNA sample transport. Jurnal Teknologi Laboratorium, 15(1), 48–58. https://doi.org/10.29238/teknolabjournal.v15i1.712

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