Pengembangan Mikroskop Dengan Mikrokontroler dan Cahaya Monokromatik Untuk Mendeteksi Parasit Malaria

Main Article Content

Ida Susanti Sarwo Handayani Riyanti Ekowatiningsih Budi Prasetyorini Endah A Yusnita Donni Agus Ardianto Sastra K Widjaya


Malaria still become one of major health burden in Indonesia especially in remote areas of east Indonesia. Golden standard of malaria parasite detection is still microscopic technique using polychromatic light source whether from halogen or natural light source. A microscopic technique has a lot of benefits but still have weaknesses, such as time-consuming and bias on the reading by microscopist, because of artifact in the image. Aims of this study were to designed malaria parasites detection tool that is robust, fast, convenient and clear by minimizing artifact on the slide. Design of this study was laboratory experimental which modified simple microscope into an automatic microscope with table movement and webcam recording using a microcontroller and monochromatic light source. The wavelength of the light sources was 402nm(blue), 532 nm (green) and 650 nm (red), the intensity of each source differed. The reading of the slide image was conducted by two certified microscopists, who read 60 images of a thick and thin slide with three different live stage of Plasmodium falciparum live, which wearing, trophozoite and schizont. This study showed that modification of microscope was succeeded with automatic movement and webcam recording, process time in one step movement and recording approximately 10 seconds or 17minutes for 100 fields of view as confirmation process. The monochromatic light source has proven to give a clear and contrast field of view when the intensities were higher than 40 mW and the certified microscopist able to identified Plasmodium falciparum parasites. Data analysis of microscopist reading used nonparametric statistic Friedman by SPSS showed that correlation between images using monochromatic and polychromatic lights have meaningless differences in a thick and thin slide. However, hemozoin as a marker of Plasmodium falciparum parasite was less detected by monochromatic light used in this study.


Download data is not yet available.

Article Details

How to Cite
Susanti, I., Handayani, S., Ekowatiningsih, R., Prasetyorini, B., A Yusnita, E., Ardianto, D., & K Widjaya, S. (2017). Pengembangan Mikroskop Dengan Mikrokontroler dan Cahaya Monokromatik Untuk Mendeteksi Parasit Malaria. Jurnal Teknologi Laboratorium, 6(2), 75-82.


[1] Arsin and A. Arsunan, Malaria Di Indonesia.Jakarta. 2012.
[2] Kemenkes RI, “Riset Kesehatan Dasar 2013,” 2013.
[3] WHO, “International Travel and Health - ITH,” 2012.
[4] J. M. Bélisle et al., “Sensitive Detection of Malaria Infection by Third Harmonic Generation Imaging,” Biophys. J., vol. 94, no. 4, pp. L26–L28, 2008.
[5] “CONTEMPORARY,” vol. 3, no. 1, 2015.
[6] M. Microscopy and S. Operating, “Microscopy Examination of Thick and Thin Blood Films for Identification of Malaria Parasites,” 2016.
[7] S. Lee and W. Lu, “Using elastic light scattering of red blood cells to detect infection of malaria parasite,” IEEE Trans. Biomed. Eng., vol. 59, no. 1, pp. 150–155, 2012.
[8] V. V. Makkapati and V. Pathangay, “Adaptive color illumination for microscopes,” 2011 Natl. Conf. Commun. NCC 2011, pp. 1–5, 2011.
[9] P. P. Kalatiku and Y. Y. Joefrie, “Pemrograman Motor Stepper Dengan Menggunakan Bahasa Pemrograman C,” no. 20 September 2015, 2011.
[10] Kemenkes, Buku saku penatalaksanaan kasus malaria. 2012.
[11] J. I. Trisnadi, “Speckle contrast reduction in laser projection displays,” Proc. Soc. Photo-Optical Instrum. Eng., vol. 4657, no. 2002, pp. 131–137, 2002.
[12] E. al Zoueo, Jeremy, “Optical_Microscope_Based_on_Multispectral_Imaging_ .pdf.” Asian network for scientific indormation, 2008.