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Biotechnology

MSC-derived exosomes ppregulate KGF and PDGF expression in a rat model of second-degree burn injury

Dewi Atika Putri Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence dewiatikaputri@yahoo.com
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Agung Putra Department of Pathological Anatomy, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence dr.agungptr@unisula.ac.id
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Titiek Sumarawati Department of Doctoral Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence titiek.sumarawati@unissula.ac.id
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Eko Setiawan Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence drekosetiawan@unissula.ac.id
Pages 310-321
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Abstract

Second-degree burn injuries necessitate efficient treatment approaches to expedite wound healing and reinstate skin integrity. Exosomes generated from mesenchymal stem cells (E-MSCs) are a promising cell-free therapeutic approach owing to their capacity to control inflammation and facilitate tissue regeneration. This research utilized a post-test-only control group experimental design with 28 male Wistar rats, randomly allocated into four groups: healthy control (G1), burn damage treated with NaCl (G2), and burn injury treated with E-MSCs at doses of 100 µL (G3) and 200 µL (G4). On the seventh day following therapy, the expression levels of keratinocyte growth factor (KGF) and platelet-derived growth factor (PDGF) were measured using RT-PCR. The expression of KGF was markedly elevated in G3 (0.65 ± 0.03) and G4 (0.92 ± 0.08) in comparison to G2 (0.32 ± 0.09; p < 0.001). Likewise, PDGF expression was significantly elevated in G3 (1.08 ± 0.24) and G4 (1.49 ± 0.19) compared to G2 (0.66 ± 0.08; p < 0.001). The results indicate a dose-dependent influence of E-MSC administration on the upregulation of KGF and PDGF, which are essential for epithelialization and angiogenesis in the wound healing process. In conclusion, E-MSCs demonstrate considerable promise as a cell-free treatment strategy for improving the healing of second-degree burns. Additional research is necessary to confirm these results and evaluate their practical relevance in clinical environments.

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How to Cite This

Putri, D. A., Putra, A., Sumarawati, T., & Setiawan, E. (2025). MSC-derived exosomes ppregulate KGF and PDGF expression in a rat model of second-degree burn injury. Jurnal Teknologi Laboratorium, 14(2), 310–321. https://doi.org/10.29238/teknolabjournal.v14i2.577

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All data generated during this study are included in this published article [and its supplementary information files].