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Biotechnology

Effects of EH-MSC–derived exosomes on oxidative stress and inflammatory markers in a UVB-induced skin damage rat model

Meri Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence merilfc81@gmail.com
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Agung Putra Department of Pathological Anatomy, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence dr.agungptr@unisula.ac.id
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Chodijah Department of Anatomy, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence chodidjah@unissula.ac.id
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Eko Setiawan Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence drekosetiawan@unissula.ac.id
Pages 322-334
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Abstract

Prolonged exposure to ultraviolet B (UVB) radiation causes persistent skin inflammation, resulting in oxidative stress, collagen breakdown, and progressive loss of collagen. Exosomes generated from mesenchymal stem cells of the umbilical cord (EH-MSCs) have surfaced as a promising cell-free therapeutic approach owing to their anti-inflammatory and antioxidant characteristics. This experimental work assessed the impact of EH-MSC exosomes on inflammatory and oxidative stress indicators in a rat model of collagen loss induced by UVB exposure. Wistar rats were categorized into five groups: healthy control (G1), UVB-exposed animals administered NaCl (G2), hyaluronic acid (G3), 200 µL of EH-MSC exosomes (G4), and 300 µL of EH-MSC exosomes (G5). Exosomes were extracted from rat umbilical cord mesenchymal stem cells via tangential flow filtration and subsequently analyzed using flow cytometry. Tumor necrosis factor-alpha (TNF-α) and glutathione peroxidase (GPx) concentrations were quantified via ELISA, and statistical evaluation was conducted employing ANOVA and Kruskal–Wallis tests. The minimal TNF-α levels were recorded in the healthy control group (72.08 ± 18.30 pg/mL), whereas the maximal values were identified in the hyaluronic acid group (216.80 ± 56.27 pg/mL). Subcutaneous delivery of EH-MSC exosomes in G4 and G5 markedly decreased TNF-α levels in comparison to the saline and hyaluronic acid groups (p < 0.05). GPx levels were maximal in the G5 group (722.60 ± 57.67 pg/mL) and minimal in the saline-treated group (46.90 ± 11.29 pg/mL). Marked disparities in GPx levels were noted among the treatment groups (p < 0.05). The results demonstrate that subcutaneous delivery of EH-MSC exosomes at dosages of 200 µL and 300 µL significantly reduces inflammation and improves antioxidant activity in UVB-induced collagen degradation. EH-MSC exosomes exhibit promise as a cell-free therapeutic strategy for alleviating UVB-induced skin damage.

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How to Cite This

Meri, Agung Putra, Chodijah, & Eko Setiawan. (2025). Effects of EH-MSC–derived exosomes on oxidative stress and inflammatory markers in a UVB-induced skin damage rat model. Jurnal Teknologi Laboratorium, 14(2), 322–334. https://doi.org/10.29238/teknolabjournal.v14i2.575

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