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Biotechnology

Hypoxia Mesenchymal Stem Cell-Derived Exosomes (EH-MSCs) enhance IGF-1 and suppress IL-1Beta in hair follicle regeneration

Evan Christian Sumarno Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence just.for.elcs@gmail.com
,
Sri Priyantini Mulyani Department of Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence sripriyantini@gmail.com
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Chodidjah Mulyani Department of Anatomy, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence chodidjah@unissula.ac.id
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Eko Setiawan Department of Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence drekosetiawan@unissula.ac.id
Pages 01-12
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Abstract

Alopecia is a condition characterized by hair loss, often caused by inflammation and disruption of growth factor pathways, such as interleukin-1 beta (IL-1Beta) and insulin-like growth factor-1 (IGF-1). This study evaluated the effects of Exosome Hypoxia Mesenchymal Stem Cells (EH-MSCs) on IL-1β and IGF-1 levels in fluconazole-induced alopecia-like Wistar rats. Using a randomized in vivo experimental design, rats were divided into five groups: a healthy control, a fluconazole-only group, a minoxidil-treated group, and two EH-MSC-treated groups with different doses (100 µg/kgBW and 200 µg/kgBW). IL-1Beta and IGF-1 levels were analyzed using ELISA. The results revealed that EH-MSCs significantly suppressed IL-1Beta levels, particularly at a dose of 200 µg/kgBW, achieving values comparable to the healthy control group. Similarly, IGF-1 levels were restored significantly, with the 200 µg/kgBW dose showing near-normal levels. These effects are attributed to the inhibition of NF-κB signaling by exosomal miR-146a and the upregulation of IGF-1 through miR-126-mediated PI3K/Akt activation. The findings highlight the dual role of EH-MSCs in reducing inflammation and promoting hair follicle regeneration. This study provides critical insights into the therapeutic potential of EH-MSCs as a cell-free alternative for treating alopecia and lays the groundwork for further research on their clinical applications.

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How to Cite This

Sumarno, E. C., Mulyani, S. P., Mulyani, C., & Setiawan, E. (2026). Hypoxia Mesenchymal Stem Cell-Derived Exosomes (EH-MSCs) enhance IGF-1 and suppress IL-1Beta in hair follicle regeneration . Jurnal Teknologi Laboratorium, 15(1), 01–12. https://doi.org/10.29238/teknolabjournal.v15i1.573

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All data generated during this study are included in this published article [and its supplementary information files].