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Biotechnology

GC-MS Analysis of phytochemical compounds from Javan olive leaves (Olea javanica) extract and bioactivity screening of antidiabetes through in silico approach

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Pages 52-63

Abstract

Diabetes is one of the most common metabolic diseases with the ninth highest mortality rate in the world. Although there are many effective hypoglycemic drugs available for the treatment of diabetes, researchers are constantly looking for more effective drugs with fewer side effects by focusing on various metabolic components such as enzymes, transporters, receptors. The Glucokinase (HGKA) enzyme, which is mainly found in the liver and pancreatic beta cells, is involved in maintaining blood glucose homeostasis. Therefore, the purpose of this study on GC-MS and in silico analysis was designed to determine the interaction between Glucokinase and the compound (ligand) of Java olive leaf extract (Olea javanica). The method used in this study was maceration with 96% ethanol to produce Java olive leaf extract, then analyzed by GC-MS using the Agilent 6980N Network GC System chromatography, then in silico analysis was carried out using the PyRx 0.9 software, pkCSM website, Biovia Discovery Studio 2019, RCSB PDB database, BIOVIA Discovery Studio software, AutoDock Vina, CABS-flex 2.0 web server, and Lipinski's rules. The results of the GC-MS analysis showed compounds in the form of 3-methylpentane, Hexane, Methylcyclopentane, Alpha-muurolene, -(-calamenene), Methyl 14-methylpentadecanoid acid, Methyl ester linoleic acid, Trans squalene, Alpha tocopherol. In silico analysis revealed a molecule that matched the target protein for the treatment of diabetes in the form of the compound -(-calamenene) which has antidiabetic activity based on molecular docking in this study.

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References

  • Markhali, F.S.; Teixeira, J.A.; Rocha CMR. Olive Tree Leaves—A Source of Valuable Active Compounds Fereshteh. processes. 2020;8(1177):1-18.
  • Cádiz-Gurrea M de la L, Pinto D, Delerue-Matos C, Rodrigues F. Olive fruit and leaf wastes as bioactive ingredients for cosmetics—a preliminary study. Antioxidants. 2021;10(2):1-18. doi:10.3390/antiox10020245
  • Al-Attar AM, Alsalmi FA. Effect of Olea europaea leaves extract on streptozotocin induced diabetes in male albino rats. Saudi Journal of Biological Sciences. 2019;26(1):118-128. doi:10.1016/j.sjbs.2017.03.002
  • Rauf A, Rashid U, Shah ZA, et al. Natural Compound from Olea ferruginea. processes. 2023;11(545):1-11.
  • Baccouri B, Rajhi I, Theresa S, Najjar Y, Mohamed SN, Willenberg I. The potential of wild olive leaves (Olea europaea L. subsp. oleaster) addition as a functional additive in olive oil production: the effects on bioactive and nutraceutical compounds using LC–ESI–QTOF/MS. European Food Research and Technology. 2022;248(11):2809-2823. doi:10.1007/s00217-022-04091-y
  • Millati A, Bahar Y, Kusumawinakhyu T. Pengaruh Sediaan Dekok Daun Zaitun (Olea europaea L.) terhadap Kadar Glukosa Darah pada Tikus Putih Galur Wistar (Rattus norvegicus) Galur Wistar Jantan yang Diinduksi Aloksan. Herb-Medicine Journal. 2019;2(2):20. doi:10.30595/hmj.v2i2.4796
  • Olmo-García L, Kessler N, Neuweger H, et al. Unravelling the distribution of secondary metabolites in olea europaea l.: exhaustive characterization of eight olive-tree derived matrices by complementary platforms (LC-ESI/APCI-MS and GC-APCI-MS). Molecules. 2018;23(10):1-16. doi:10.3390/molecules23102419
  • Kandeepan C, Sabitha M, Parvathi K, et al. Phytochemical Screening, GCMS Profile, and In-silico properties of Bioactive Compounds in Methanolic Leaf Extracts of Moringa oleifera. Journal of Drug Delivery and Therapeutics. 2022;12(2):87-99. doi:10.22270/jddt.v12i2.5250
  • Kesuma D, Siswandono S, Purwanto BT, Hardjono S. Uji in silico Aktivitas Sitotoksik dan Toksisitas Senyawa Turunan N-(Benzoil)-N’- feniltiourea Sebagai Calon Obat Antikanker. JPSCR?: Journal of Pharmaceutical Science and Clinical Research. 2018;3(1):1. doi:10.20961/jpscr.v3i1.16266
  • Warnis M, Aprilina LA, Maryanti L. Pengaruh Suhu Pengeringan Simplisia Terhadap Kadar Flavonoid Total Ekstrak Daun Kelor (Moringa oleifera L.). Seminar Nasional Kahuripan. 2020;(Seminar Nasional Kahuripan (SNapan) 2020):264-268.
  • Widyananda MH, Wicaksono ST, Rahmawati K, et al. A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line. Scientifica. 2022;2022:1-17. doi:10.1155/2022/9130252
  • Tripathy D, Nayak BS, Mohanty B, Mishra B. Solid dispersion: A technology for improving aqueous solubility of drug. J Pharm Adv Res. 2019;2(7):577-586.
  • Wargasetia TL, Ratnawati H, Widodo N, Widyananda MH. Bioinformatics Study of Sea Cucumber Peptides as Antibreast Cancer Through Inhibiting the Activity of Overexpressed Protein (EGFR, PI3K, AKT1, and CDK4). Cancer Informatics. 2021;20:1-11. doi:10.1177/11769351211031864
  • Aini NS, Kharisma VD, Ansori A, et al. Bioactive compounds screening of Rafflesia sp. and Sapria sp. (Family: Rafflesiaceae) as anti-SARS-CoV-2 via tetra inhibitors: An in silico research. Journal of Pharmacy and Pharmacognosy Research. 2023;11(4):611-624. doi:10.56499/jppres23.1620_11.4.611
  • Chandel V, Tripathi G, Nayar SA, Rathi B, Kumar A, Kumar D. In silico identification and validation of triarylchromones as potential inhibitor against main protease of severe acute respiratory syndrome coronavirus 2. Journal of biomolecular structure & dynamics. 2022;40(19):8850-8865. doi:10.1080/07391102.2021.1918255
  • Nusantoro YR, Fadlan A. Analisis Sifat Mirip Obat, Prediksi ADMET, dan Penambatan Molekular Isatinil-2-Aminobenzoilhidrazon dan kompleks logam transisi Co(II), Ni(II), Cu(II), Zn(II) Terhadap BCL2-XL. Akta Kimia Indonesia. 2020;5(2):114. doi:10.12962/j25493736.v5i2.7881
  • Maulida MZ, Ummahati D, Muna LN, et al. SIFAT TOKSISITAS SENYAWA AKTIF DALAM JAHE MERAH ( Zingiber. Journal of pharmaceutical care and sciences. 2023;4(1):90-98. doi:10.33859/jpcs.v4i1.454
  • Putu N, Primananda W, Hanggaresty D, Adi A, Putra P, Warditiani NK. Docking Molekuler??-Sitosterol Dengan Protein Dipeptidylpeptidase-4 (Dpp-4) Sebagai Antiheperglikemia Secara in Silicodocking Molekuler??-Sitosterol Dengan Protein Dipeptidylpeptidase-4 (Dpp-4) Sebagai Antiheperglikemia Secara in Silico. CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry). 2022;10(1):10-16.
  • Laoung-On J, Jaikang C, Saenphet K, Sudwan P. Effect of Nelumbo nucifera Petals Extract on Antioxidant Activity and Sperm Quality in Charolais Cattle Sperm Induced by Mancozeb. Plants (Basel, Switzerland). 2022;11(5). doi:10.3390/plants11050637
  • Anthony Ammal SM, Sudha S, Rajkumar D, Baskaran A, Krishnamoorthy G, Anbumozhi MK. In Silico Molecular Docking Studies of Phytocompounds From Coleus Amboinicus Against Glucokinase. Cureus. 2023;15(2):6-11. doi:10.7759/cureus.34507
  • Khan G, Jain S. In silico-qsar modelling of predicted glucokinase - glucokinase regulatory protein inhibitors against diabetes Section A-Research paper. ResearchGate. 2023;12(May):930-957.
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How to Cite This

Fitri, L., Wulandari, A. D., Sumaryono, T., Haibaturrahma, N. F. A., Agustina, B. N., & Tamam, M. B. (2024). GC-MS Analysis of phytochemical compounds from Javan olive leaves (Olea javanica) extract and bioactivity screening of antidiabetes through in silico approach . Jurnal Teknologi Laboratorium, 13(1), 52–63. https://doi.org/10.29238/teknolabjournal.v13i1.467

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