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Food and Drink Chemistry

Anti-hyperuricemia effect of ethanol and n-hexane fractions of celery leaf and stem in Wistar rats

Agnes Fani Cahyani Hura Magister Program of Biomedical Sciences, Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia, Indonesia Correspondence agnesfany28@gmail.com
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Maya Sari Mutia Medical Education Program, Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia, Indonesia Correspondence mayasarimutia@unprimdn.ac.id
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Refi Ikhtiari Magister Program of Biomedical Sciences, Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia, Indonesia Correspondence refiikhtiari@unprimdn.ac.id
Pages 96-108
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Abstract

This study investigated the antihyperuricemic effects of ethanol and n-hexane fractions derived from celery (Apium graveolens L.) leaves and stems in potassium oxonate-induced Wistar rats. Celery plant materials were subjected to extraction and subsequent fractionation using ethanol and n-hexane. Phytochemical profiling using gas chromatography–mass spectrometry (GC–MS) identified the presence of phenolic compounds across all fractions. Serum uric acid levels were measured before and after induction, followed by treatment with allopurinol and the respective celery fractions. Statistical analysis revealed significant differences in uric acid levels among treatment groups after intervention. In vitro xanthine oxidase (XO) inhibition was assessed using UV–Vis spectrophotometry, with allopurinol demonstrating the highest inhibitory activity, followed by the ethanol fraction of celery stems. Histopathological evaluation of renal tissues showed glomerular necrosis in the untreated hyperuricemic group, while varying degrees of renal damage persisted in groups treated with celery fractions, particularly at higher doses of leaf extracts. Overall, the findings suggest that ethanol and n-hexane fractions of celery possess antihyperuricemic potential, likely mediated through XO inhibition, although their renal protective effects appear limited under the conditions tested.

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How to Cite This

Hura, A. F. C., Mutia, M. S., & Ikhtiari, R. (2026). Anti-hyperuricemia effect of ethanol and n-hexane fractions of celery leaf and stem in Wistar rats. Jurnal Teknologi Laboratorium, 15(1), 96–108. https://doi.org/10.29238/teknolabjournal.v15i1.415

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