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The wound healing effect of Morinda citrifolia leaf extract and biomolecular analysis on inflammation and proliferation stages in Wistar rats.

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Pages 52-59


This research aims to evaluate the wound healing effect of noni’s leaf extract (Morinda citrifolia L.) based on phytochemical analysis, the number of fibroblast cells, wound diameter, healing time, TNF-a and IL-1 at inflammation stage, PDGF and TGF-b at proliferation stage. This study is a true experimental with a post-test-only control group design. We divided 25 male Wistar rats into five groups; positive control (with povidone-iodine), negative control (with no treatment), treated by NLEE 20%, 25%, and 30%. NLEE has good physical characteristics based on the value of ethanol soluble extract, water-soluble extract, water content, ash content, and acid insoluble ash content. GCMS analysis showed bioactive compounds such as N-ethyl hydrazine carbothioamide (31.57%), 2-furancarboxaldehyde 5-hydroxymethyl (15,64%), 2E-3,7,11,15-tetramethyl-2-hexadecene-1-of (7.08%), 3,5-dihydroxy-6-methyl-2,3-dihydro-4H-pyran-4-one (4.29%), D:C-friedooleana-7,9(11)-dien-3-ol,3beta (4.04%), while the rest are considered as the decanoic acids methyl ester, piperazine (3%) and vitamin E (1%). We observed the slight effects of NLEE 20%, 25%, and 30% on the number of fibroblasts, wound diameter, healing time, TNF-a, IL-1, PDGF, and TGF-b. The optimum concentration of NLEE at any treatment was 20%. However, there were no significant differences between groups based on a two-way ANOVA analysis.

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How to Cite This

Grace Noviyanthi Sinambela, Tandanu, E., & Ikhtiari, R. (2022). The wound healing effect of Morinda citrifolia leaf extract and biomolecular analysis on inflammation and proliferation stages in Wistar rats. Jurnal Teknologi Laboratorium, 11(2), 52–59. https://doi.org/10.29238/teknolabjournal.v11i2.369

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