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Hematology

Erythrocyte index of residents exposed to lead in Tambaklorok, Semarang, Indonesia

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Pages 151-158

Abstract

Erythrocyte index can form erythrocyte morphology in the peripheral blood smear preparation. Lead exposure in Tambaklorok exceeds the threshold that affects anemia. The purpose of this study was to determine the erythrocyte index confirmation with erythrocyte morphology in the peripheral blood smear preparation. A cross-sectional study was conducted in 2month in Tambaklorok Semarang residents and 104 samples were taken using the purposive technique. Erythrocyte index was measured using the hematological analyzer and erythrocyte morphology in the peripheral blood smear preparation using Giemsa painting. Erythrocyte index confirmation with the peripheral blood smear preparation was analyzed using the Gamma statistical relationship test. The results showed that the erythrocyte index value was mostly in the normal category, i.e., MCH 68 (64.4%), MCHC 61 (58.6%) and MCH 58 (56%) and below normal category were MCH 45 (42.95%), MCHC 41 (39.4%), and MCV 36 (34.3%). Erythrocyte morphology was mostly hypochromic, namely 46 (44.23%), normochrome 40 (38.46%), and hyperchrome 18 (17.3%). Relationship of MCH with erythrocyte color p-value 0.037 with size p-value 0.038. Conclusion of erythrocyte index confirmation, especially MCH with the peripheral blood smear preparation, there was a match on the color and size of erythrocytes, while the MCV and MCHC values had no significant relationship.

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References

1. Budiwiyono Y, Rachmawati B, Hendrianingtyas M. Perbedaan index eritrosit pada pasien anemia gagal ginjal kronik dan thalassemia mayor. J Kedokt Diponegoro. 5(4):1911–22.
2. Daulay MCG. Morfologi Eritrosit Pada Sediaan Apus Darah Tepi (SADT) Sampel Dengan Hasil Pemeriksaan One Tube Osmotic Fragility Test (OTOFT) Positif. Skripsi. 2013;1`12.
3. Sarma PR. Red Cell Indices. Vol 30.; 1990. http://www.ncbi.nlm.nih.gov/pubmed/21250103.
4. Rubiyanti A. Morfologi eritrosit pada penderita anemia defisiensi besi metode apusan darah tepi.
5. Salim Y, Sukartini N, Setiawati A. Erythrocyte Indices to Differentiate Iron Deficiency Anemia from Β Trait Thalassemia (Indeks Eritrosit untuk Membedakan Anemia Defisiensi Besi dengan Thalassemia β Trait). Indones J Clin Pathol Med Lab. 2018;23(1):50. doi:10.24293/ijcpml.v23i1.1184.
6. Chiu Y-W, Liu T-Y, Chuang H-Y. The Effects of Lead Exposure on the Activities of δ-Aminolevulinic Acid Dehydratase with the Modification of the Relative Genotypes. Pirrone N, ed. E3S Web Conf. 2013;1:26005. doi:10.1051/e3sconf/20130126005.
7. Hsieh N-H, Chung S-H, Chen S-C, et al. Anemia risk in relation to lead exposure in lead-related manufacturing. BMC Public Health. 2017;17(1):389. doi:10.1186/s12889-017-4315-7.
8. Santosa B, Iswara A, Ernanto AR. IR-bagendit leaves water extract as preventing agent in hematopoiesis, degeneration and necrosis in kidney tubulus of lead-exposed rats. Pakistan J Med Heal Sci. 13(3):899–904.
9. Multipalli A. Lead hepatotoxicity & potential health effects. Indian J Med Res. 126(6):518–27.
10. Branch C. Study on the possible effects on human health and the environment in Asia and the Pacific of the trade of products containing lead, cadmium and mercury. In: UNITED NATIONS ENVIRONMENT PROGRAMME CHEMICALS BRANCH. https://ipen.org/sites/default/files/documents/health_effects_heavy_metals_asia_pacific-en.pdf.
11. Supriyantini E, Soenardjo N. Kandungan logam berat timbal (Pb) dan tembaga (Cu) pada akar dan buah Mangrove Avicennia marina di perairan Tanjung Emas Semarang. J Kelaut Trop. 2016;18(2):98–106. doi:10.14710/jkt.v18i2.520.
12. Nana Kariada. Tingkat Kualitas Udara Di Jalan Protokol Kota Semarang. J Sains dan Teknol. 2011;9(2):111–20. doi:10.15294/sainteknol.v9i2.5532.
13. Marianti A, Prasetya AT. Rambut Sebagai Bioindikator Pencemaran Timbal Pada Penduduk Di Kecamatan Semarang Utara. Biosaintifika J Biol Biol Educ. 2013;5(1):10–5. doi:https://doi.org/10.15294/biosaintifika.v5i1.2568.
14. Lemeshow S, David W. Jr K, J. Adequacy of Sample Size in Health Studies. :41–57. https://www.academia.edu/39511442/Adequacy_of_Sample_Size_in_Health_Studies.
15. Khanam S, Begum N, Hoque AE. Relationship of Hemoglobin, Packed Cell Volume and Total Count of RBC with the Severity of Chronic Renal Failure. Chattagram Maa-O-Shishu Hosp Med Coll J. 2013;12(2). doi:10.11566/cmoshmcj.v12i2.36.
16. Adewoyin AS, Nwogoh B. Peripheral blood film - a review. Ann Ibadan Postgrad Med. 2014;12(2):71-79. http://www.ncbi.nlm.nih.gov/pubmed/25960697.
17. Malka R, Delgado FF, Manalis SR, Higgins JM. In Vivo volume and hemoglobin dynamics of human red blood cells. Beard DA, ed. PLoS Comput Biol. 2014;10(10):e1003839. doi:10.1371/journal.pcbi.1003839.
18. Otto JM, Plumb JOM, Clissold E, et al. Hemoglobin concentration, total hemoglobin mass and plasma volume in patients: implications for anemia. Haematologica. 2017;102(9):1477-1485. doi:10.3324/haematol.2017.169680.
19. Buttarello M. Laboratory diagnosis of anemia: are the old and new red cell parameters useful in classification and treatment, how? Int J Lab Hematol. 2016;38:123-132. doi:10.1111/ijlh.12500.
20. Singh M, Gautam A. To evaluate anaemia by erythrocyte indices, red cell distribution width and haemoglobin electrophoresis with special reference to thalassemia in paediatric age group. J Evol Med Dent Sci. 2015;04(11):1755-1764. doi:10.14260/jemds/2015/251.
21. Ogun AS, Joy N V., Valentine M. Biochemistry, Heme Synthesis.; 2020. https://www.ncbi.nlm.nih.gov/books/NBK537329/.
22. Santosa B. Variasi dosis suplementasi Zn memperbaiki hematopoesis pada tikus yang terpajan Plumbum (Pb). :37–45.
23. Santosa B, Sunoko HR, Sukeksi A. Aqueous IR Bagendit rice leaf extract decreases reticulocyte count in lead-exposed rats. Universa Med. 2018;37(1):57. doi:10.18051/UnivMed.2018.v37.57-64.
24. Santoso B, Subagio HW, Suromo L, Sunomo HR. Zinc supplementation improves heme biosynthesis in rats exposed to lead. Universa Med. 2016;34(1):3. doi:10.18051/UnivMed.2015.v34.3-9.
25. Agency for Toxic Substances and Disease Registry (ATSDR). Lead Toxicity Case Studies in Environmental Medicine. WB2832:1–182. https://www.atsdr.cdc.gov/csem/lead/docs/CSEM-Lead_toxicity_508.pdf.
26. Sassa S. Toxic Effects of Lead, with Particular Reference to Porphyrin and Heme Metabolism. In: Heme and Hemoproteins. Berlin, Heidelberg: Springer Berlin Heidelberg; 1978:333-371. doi:10.1007/978-3-642-66763-3_11.
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How to Cite This

Santosa, B., Anggraini, H., Mawarni, I. D., Muliatun, N., Nugroho, A., Syarah, U., & Risqoeni, S. A. (2020). Erythrocyte index of residents exposed to lead in Tambaklorok, Semarang, Indonesia. Jurnal Teknologi Laboratorium, 9(2), 151–158. https://doi.org/10.29238/teknolabjournal.v9i2.213

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