Optimasi Waktu Produksi Metabolit Sekunder dan Skrining Aktivitas Antibakteri Isolat Actinomycetes Rizosfer Tanaman Tin (Ficus carica)
Some Actinomycetes isolates of a tin plant (Ficus carica L.) have been obtained, namely T24M, T18, T19, T24, T25, T34, T37, T41, and T43. The aim of this study was to optimize the production of secondary metabolites (antibiotics) and screening antibacterial activity against Methicillin-Resistant Staphylococcus aureus (MRSA) from the Actinomycetes isolate of the tin rhizosphere. The study was performed with test an activity of the culture fluid from Actinomycetes isolate against MRSA by the well method. The result of optimization secondary metabolite production showed that the second day was the best incubation time to harvest antibiotics. The results showed that bacterial isolates of T24M produced antibiotics that could inhibit MRSA growth.
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2. Parungao M, Maceda E, Villano M. Screening of Antibiotic-Producing Actinomycetes from Marine, Brackish and Terrestrial Sediments of Samal Island, Philippines. J Res Sci Comput Eng. 2007;4(3):29-38.
3. Sulistyani N, Muhlis M, Kustanti N, Erinto E, Aquina H, Zainab. Studi Resistensi Staphylococcus aureus yang diisolasi dari Limbah Cair Beberapa Rumah Sakit terhadap Antibotika. In: Prosiding Seminar Nasional Kesehatan Lingkungan Untuk Mewujudkan Sehat Jasmani Rohani Bagi Anak Bangsa. ; 2009.
4. Ogunmwonyi I., Mazomba N, Mabinya L, et al. Studies on the culturable marine actinomycetes isolated from the Nahoon beach in the Eastern Cape Province of South Africa. Afr J Microbiol Res. 2010;4(21):2223-2230.
5. Genilloud O, GonzÃ¡lez I, Salazar O, MartÃn J, Tormo JR, Vicente F. Current approaches to exploit actinomycetes as a source of novel natural products. J Ind Microbiol Biotechnol. 2011;38(3):375-389. doi:10.1007/s10295-010-0882-7
6. Berdy J. Bioactive microbial metabolites. A Pers view J Antibiot. 2005;58(1):1-26.
7. Rollins D, Joseph S. Actinomycetes. University of Maryland.
8. Ambarwati, Gama A. Isolasi Actinomycetes Dari Tanah Sawah Sebagai Penghasil Antibiotik. J Penelit Sains Teknol. 2009;10(2):101-111.
9. Busti E, Monciardini P, Cavaletti L, et al. Antibiotic-producing ability by representatives of a newly discovered lineage of actinomycetes. Microbiology. 2006;152:675-683.
10. Kurniasari R, Sulistyani N. Anactinomycetes (Isolate T34) as Antibiotic Producer Againts, Staphylococcus aureus and Bioautography Analysis. In: Proceeding of International Safety Management of Central Cytotoxic Reconstitution. ; 2013:83-88.
11. Todar K. Nutrion and Growth of Bacteria in Todarâ€™s Online Textbook of Bacteriology.; 2007.
12. Ali A. Title Skrining dan karakterisasi parsial senyawa antifungi dari Actinomycetes asal limbah padat sagu terdekomposisi. Berk Penel Hayati. 2009;14:219-225.
13. Hamid A, Ariffin S, Mohamad S. Identification And Optimal Growth Conditions Of Actinomycetes Isolated From Mangrove Environment. Malaysian J Anal Sci. 2015;19(4):904-910.
14. Augustine S, Bhavsar S, Kapadis B. Production of growth dependent metabolite active agains dermatophytes by Streptomyces rochei Ak 39. Indian J Med. 2005;121:164-170.
15. Oskay M. Antifungal and antibacterial compounds from Streptomyces strains. African J Biotechnol. 2009;8(13):3007-3017.
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