Jurnal Teknologi Laboratorium https://www.teknolabjournal.com/index.php/Jtl <p style="text-align: justify;"><em>Jurnal Teknologi Laboratorium</em>, with registered number ISSN 2<a href="http://issn.pdii.lipi.go.id/issn.cgi?daftar&amp;1369726769&amp;1&amp;&amp;" target="_blank" rel="noopener">338-5634&nbsp;(print)</a>, <a href="http://issn.pdii.lipi.go.id/issn.cgi?daftar&amp;1493892253&amp;1&amp;&amp;" target="_blank" rel="noopener">2580-0191&nbsp;(online)</a>&nbsp;is a scientific journal published by&nbsp;Poltekkes Kemenkes Yogyakarta. The journal <span class="st">registered in the CrossRef system </span><span class="il">with </span><strong><span class="st">Digital Object Identifier </span><span class="il">(DOI)</span> prefix <a href="https://search.crossref.org/?q=2580-0191&amp;publication=Jurnal+Teknologi+Laboratorium" target="_blank" rel="noopener">10.29238.</a>&nbsp;</strong>&nbsp;The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of medical laboratory sciences.&nbsp; Every article that goes to the editorial staff will be selected through <strong>Initial Review</strong> processes by Editorial Board. Then, the articles will be sent to the Mitra Bebestari/ peer reviewer and will go to the next selection by Double-Blind<strong> Preview Process</strong>. After that, the articles will be returned to the authors to revise. These processes take a month for a maximum time. In each manuscript, Mitra Bebestari/ peer reviewer will be rated from the substantial and technical aspects. The final decision of articles acceptance will be made by Editors according to Reviewers comments.&nbsp;Mitra Bebestari/ peer reviewer that collaboration with <em>Jurnal Teknologi Laboratorium</em>&nbsp;is the experts in the medical laboratory technology&nbsp;area and issues around it.</p> <p style="text-align: justify;"><em>Jurnal Teknologi Laboratorium</em> particularly focuses on the main problems in the development of the sciences of medical laboratory&nbsp;areas. It covers the parasitology, bacteriology, virology, haematology, clinical chemistry, toxicology, food and drink chemistry.</p> <p style="text-align: justify;">In order to further internationalize our journal, Jurnal Teknologi Laboratorium has decided to change the Indonesian language to<strong> accept with English or Indonesian</strong> starting from volume 7 issue 1 March 2018. Due to this change, the article guideline of Jurnal Teknologi Laboratorium will be renewed accordingly.</p> <p style="text-align: justify;"><strong>Please read these guidelines carefully</strong>. &nbsp;Authors who want to submit their&nbsp;manuscript to the editorial office of&nbsp;<em>Jurnal Teknologi Laboratorium</em>&nbsp;should obey the writing guidelines. If the&nbsp;manuscript submitted is not appropriate with the guidelines or written in a different format, <strong>it will&nbsp;BE REJECTED</strong> by the editors before further reviewed. The editors will only accept the manuscripts which meet the assigned format.</p> <table style="background-color: #ccffff; margin: 20px auto 15px;" width="100%"> <tbody> <tr> <td style="width: 100%; text-align: center;"> <h2 style="margin-top: 15px; text-transform: uppercase;">Statistics</h2> </td> </tr> <tr> <td style="width: 581px;"> <p style="padding-left: 30px;"><strong>Volume 7 No 1. 2018:</strong></p> <p style="padding-left: 30px;">Articles Received: 13; Accepted: 8; Rejected: 5; Published: 5; Retracted: 0</p> <p><strong>&nbsp; &nbsp; &nbsp; &nbsp;Volume 7 No 2. 2018:</strong></p> <p>&nbsp; &nbsp; &nbsp; &nbsp;Articles Received: 11; Accepted: 6; Rejected: 5; Published: 5; Retracted: 0</p> </td> </tr> </tbody> </table> POLTEKKES KEMENKES YOGYAKARTA en-US Jurnal Teknologi Laboratorium 2338-5634 <p style="text-align: justify;">Publishing your paper with Jurnal Teknologi Laboratorium&nbsp;(JTL)&nbsp;means that the author or authors retain the copyright in the paper. JTL&nbsp;granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by JTL&nbsp;in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.</p> <p style="text-align: justify;">JTL&nbsp;journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under&nbsp;a&nbsp;<a href="http://creativecommons.org/licenses/by-sa/4.0/" rel="license">Creative Commons Attribution-ShareAlike 4.0 International License</a>.&nbsp;This license lets others remix, transform, and build upon the material&nbsp;for any purpose, even commercially.</p> <p style="text-align: justify;">JTL&nbsp;journal Open Access articles are distributed under this <strong><a href="http://creativecommons.org/licenses/by-sa/4.0/" rel="license">Creative Commons Attribution-ShareAlike 4.0 International License</a>&nbsp;(CC BY-SA).</strong>&nbsp;Articles can be read and shared for All&nbsp;purposes under the following conditions:</p> <ul> <li class="show" style="text-align: justify;"><em>BY:</em>&nbsp;You must give <a id="appropriate_credit_popup" class="helpLink" href="https://creativecommons.org/licenses/by-sa/4.0/#">appropriate credit</a>, provide a link to the license, and&nbsp;<a id="indicate_changes_popup" class="helpLink" href="https://creativecommons.org/licenses/by-sa/4.0/#">indicate if changes were made</a>. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.</li> <li class="show" style="text-align: justify;"><em>SA:</em>&nbsp; If you remix, transform, or build upon the material, you must distribute your contributions under the <a id="same_license_popup" class="helpLink" href="https://creativecommons.org/licenses/by-sa/4.0/#">same license</a> as the original.</li> </ul> Antibiotic Resistant and Plasmid Conjugative Study of Salmonella typhi https://www.teknolabjournal.com/index.php/Jtl/article/view/134 <p style="text-align: justify;">The emergence of multi-drug resistant (MDR) bacteria has endangered the efficacy of antibiotics treatment of pathogenic bacteria worldwide. The aim of this research was to investigate the incidence of Salmonella enterica serovar Typhi in Duhok city, Iraq. Specimens of blood and stool were recruited from 267 patients. S. Typhi isolates were diagnosed depending on morphology, biochemical and serological tests. S. Typhi isolates were tested for their antibiotic resistance. Multi-drug resistant S. Typhi isolates were conjugated with E. coli HB101. The plasmid profile of transconjugants was investigated. 15/267 (5.6%) S. Typhi isolates were identified. Based on their biochemical tests, S. Typhi isolates were categorized into two biotypes (I, 26.66% and II, 73.33%). Four resistance patterns were observed. The resistant pattern to ampicillin and tetracycline was the higher (46.6%). Conjugation experiment showed that all antibiotic markers were transferred from S. Typhi to E. coli HB101 with a conjugation frequency of (0.38×10-5). 13.3% of the S. Typhi isolates were multi-drug-resistant resistant and had two small plasmids. Transconjugants E. coli acquired the resistance from the multi-drug resistant S. Typhi. Antibiotics treatment of the pathogens could be hindered by the constant rise of multi-drug-resistant. Further studies are needed to study the mobile genetic elements and their contribution to antibiotics resistance.</p> Waleed Haji Saeed Akreyi Samira Younis Yousif Mahde Assafi ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2018-09-05 2018-09-05 7 2 38 45 10.29238/teknolabjournal.v7i2.134 Antibacterial Activity of Karika Dieng Peels Againts Shigella flexneri and Escherichia coli https://www.teknolabjournal.com/index.php/Jtl/article/view/137 <p>Karika is an endemic plant of Dieng Plateau. The leaf of Karika has been studied to have antibacterial activity against bacteria that cause diarrhea, while the peels are removed or used as a mixture of animal feed. This study aims to determine the antibacterial activity of Karika peels against bacteria that cause poisoning, <em>i.e </em><em>Shigella flexneri</em>, and <em>Escherichia coli</em>. The sample fractionated to obtain the fraction of n-hexane and ethyl acetate. The fractions were tested for their antibacterial activity against <em>Shigella flexneri</em> and <em>Escherichia coli</em> by the cup-plate method. The results showed that the ethyl acetate fraction (ethyl acetate 50%) had the highest inhibition on <em>Shigella flexneri</em> and <em>Escherichia coli</em> compared with another concentration. Based on the study can be concluded that the peels of Karikahas antibacterial activity against <em>Shigella flexneri</em> and <em>Escherichia coli</em>.</p> Dhiah Novalina Sugiyarto Sugiyarto Ari Susilowati ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2018-12-31 2018-12-31 7 2 53 60 10.29238/teknolabjournal.v7i2.137 Potential of Carica Pubescens Leaf Extract as Alternative Antidiare Bacteria for Vibrio cholerae and Shigella dysentriae https://www.teknolabjournal.com/index.php/Jtl/article/view/138 <p><em>Acute diarrhea is one of the main causes of morbidity and mortality. A people are starting to choose traditional medicines for alternative therapy. Traditional medicines or herbal medicines are considered safer and do not have side effects such as chemical drugs. The purpose of this study was to determine the anti-diarrhea effect of Carica pubescens leaf extract on the bacteria Vibrio cholerae and Shigella dysentriae. This study was conducted by testing the activity of Vibrio cholerae and Shigella dysentriae bacteria on Carica pubescens leaf extract with a well method, which results can be seen from the formation of inhibitory zones. The data obtained were processed using Two Way ANOVA test statistics. The results showed that the leaves extract of Carica Pubescens concentration of 100% had the best therapeutic effect because it had the greatest inhibitory power on the bacteria Vibrio cholerae and Shigella dysentriae.</em></p> Tri Dyah Astuti Wahid Syamsul Hadi ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2018-12-31 2018-12-31 7 2 61 69 10.29238/teknolabjournal.v7i2.138 Development of Oxgall Preparation as Clinical Sample for Detection of Salmonella typhi with Real-time PCR Method https://www.teknolabjournal.com/index.php/Jtl/article/view/127 <p>Typhoid fever is a significant public health burden in low-income countries caused by Salmonella enterica serotype typhi (<em>S.typhi</em>). Clinical manifestations of typhoid fever are varied and non-specific, making the diagnosis difficult. Using oxgall for pre-incubation as a selective culture medium before amplification of&nbsp;<em>Real-time&nbsp;</em>PCR (<em>RT-PCR</em>) in&nbsp;<em>wholeblood</em>&nbsp;produces a fast and sensitive diagnostic. The purpose of this study was to know the performance oxgall-precultured <em>Real-time </em>PCR for detection of <em>Salmonella sp.</em>. Prior to the sample process, spike method optimization was performed to find out that the reagents were well used for clinical specimens. In the sample process, blood samples from 30 Widal-positive patients were collected for this study . Venous blood samples from typhoid fever patients were taken on the day of diagnosis; 5 ml for blood culture, and 5 ml for RT-PCR. The bacteria were grown in <em>oxgall</em> 10% (standard microbiological laboratory clinics) and incubated for 6 hours (37° C) before bacterial DNA was isolated for RT-PCR detection. The results showed that reagen of&nbsp; RT-PCR is good used for a clinical sample and a blood culture was better than RT-PCR using oxgall (positive blood culture results over 24 hours). This suggests that there is a need for further research on the duration of incubation and <em>oxgall</em> concentrations in RT-PCR and the selection of clinical samples.</p> Annisa Pratiwi Gunawan Ai Djuminar Ernawati Ernawati Lidya Chaidir ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2018-12-31 2018-12-31 7 2 70 77 10.29238/teknolabjournal.v7i2.127 The Presence of Methanol in Alcoholic Beverages Analyzed Using Qualitative Method https://www.teknolabjournal.com/index.php/Jtl/article/view/117 <p style="text-align: justify;">An alcoholic beverage contains ethyl alcohol or ethanol (C2H5OH)that is produced from fermentation or distillation of sugars. In many cases, either producers or consumers add ethanol with denaturated alcohol containing methanol (CH3OH) as an additive. Methanol can cause blindness and induce comas, and it is deadly in high doses. This study aimed at investigating the presence of methanol or methyl alcohol in alcoholic beverages sold in Palembang, Indonesia. Seventeen samples collected from small shops and supermarkets were taken by accidental sampling. A chromotropic acid method was used to examine the presence of methanol. The results showed that there were 18% of the samples was positive, and 82% was negative. Based on alcohol content, the research showed that all (100%) samples of group A were negative; 33% of group B was positive, and 33% of group C was positive. The study indicated that methanol was still present in alcoholic drinks sold in markets. The government should inform the society that denatured alcohol contains methanol and, therefore, should not be feasible to consume.</p> Diah Navianti Muhammad Ihsan Tarmizi Sinta Nur Holifah ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2018-09-05 2018-09-05 7 2 46 52 10.29238/teknolabjournal.v7i1.117