Jurnal Teknologi Laboratorium https://www.teknolabjournal.com/index.php/Jtl <p style="text-align: justify;"><em>Jurnal Teknologi Laboratorium</em>, with registered number ISSN 2<a href="http://issn.pdii.lipi.go.id/issn.cgi?daftar&amp;1369726769&amp;1&amp;&amp;" target="_blank" rel="noopener">338-5634&nbsp;(print)</a>, <a href="http://issn.pdii.lipi.go.id/issn.cgi?daftar&amp;1493892253&amp;1&amp;&amp;" target="_blank" rel="noopener">2580-0191&nbsp;(online)</a>&nbsp;is a scientific journal published by&nbsp;Poltekkes Kemenkes Yogyakarta. The journal <span class="st">registered in the CrossRef system </span><span class="il">with </span><strong><span class="st">Digital Object Identifier </span><span class="il">(DOI)</span> prefix <a href="https://search.crossref.org/?q=2580-0191&amp;publication=Jurnal+Teknologi+Laboratorium" target="_blank" rel="noopener">10.29238.</a>&nbsp;</strong>&nbsp;The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of medical laboratory sciences.&nbsp; Every article that goes to the editorial staff will be selected through <strong>Initial Review</strong> processes by the Editorial Board. Then, the articles will be sent to the peer reviewer and will go to the next selection by Double-Blind<strong> Peer - review Process</strong>. After that, the articles will be returned to the authors to revise. These processes take 6 - 7 months for maximum time. In each manuscript, the peer reviewer will be rated from the substantial and technical aspects. The final decision of article acceptance will be made by Editors according to the Reviewer's comments. The reviewer that collaboration with <em>Jurnal Teknologi Laboratorium</em>&nbsp;is the experts in the medical laboratory technology&nbsp;area and issues around it.</p> <p style="text-align: justify;"><em>Jurnal Teknologi Laboratorium</em> particularly focuses on the main problems in the development of the sciences of medical laboratory&nbsp;areas. It covers parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food, and drink chemistry <strong>but for special issues</strong>, it is possible to receive another area of health like epidemiology, etc.</p> <p style="text-align: justify;">In order to further internationalize our journal, Jurnal Teknologi Laboratorium has decided to change the Indonesian language to<strong> accept the English </strong>language only, since 2019.</p> <p style="text-align: justify;"><strong>Please read these guidelines carefully</strong>. &nbsp;Authors who want to submit their&nbsp;manuscript to the editorial office of&nbsp;<em>Jurnal Teknologi Laboratorium</em>&nbsp;should obey the writing guidelines. If the&nbsp;manuscript submitted is not appropriate with the guidelines or written in a different format, <strong>it will&nbsp;BE REJECTED</strong> by the editors before further reviewed. The editors will only accept the manuscripts which meet the assigned format.</p> <table style="background-color: #ccffff; margin: 20px auto 15px;" width="100%"> <tbody> <tr> <td style="width: 100%; text-align: center;"> <h2 style="margin-top: 15px; text-transform: uppercase;">Statistics</h2> </td> </tr> <tr> <td style="width: 581px;"> <p style="padding-left: 30px;"><strong>Volume 8 No 1. 2019:</strong></p> <p style="padding-left: 30px;">Articles Received: 15; Accepted: 7; Rejected: 7; Published: 6; Retracted: 0</p> <p><strong>&nbsp; &nbsp; &nbsp; &nbsp;Volume 7 No 2. 2018:</strong></p> <p>&nbsp; &nbsp; &nbsp; &nbsp;Articles Received: 11; Accepted: 6; Rejected: 5; Published: 5; Retracted: 0</p> </td> </tr> </tbody> </table> POLTEKKES KEMENKES YOGYAKARTA en-US Jurnal Teknologi Laboratorium 2338-5634 <p style="text-align: justify;">Publishing your paper with Jurnal Teknologi Laboratorium&nbsp;(JTL)&nbsp;means that the author or authors retain the copyright in the paper. JTL&nbsp;granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by JTL&nbsp;in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.</p> <p style="text-align: justify;">JTL&nbsp;journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under&nbsp;a&nbsp;<a href="http://creativecommons.org/licenses/by-sa/4.0/" rel="license">Creative Commons Attribution-ShareAlike 4.0 International License</a>.&nbsp;This license lets others remix, transform, and build upon the material&nbsp;for any purpose, even commercially.</p> <p style="text-align: justify;">JTL&nbsp;journal Open Access articles are distributed under this <strong><a href="http://creativecommons.org/licenses/by-sa/4.0/" rel="license">Creative Commons Attribution-ShareAlike 4.0 International License</a>&nbsp;(CC BY-SA).</strong>&nbsp;Articles can be read and shared for All&nbsp;purposes under the following conditions:</p> <ul> <li class="show" style="text-align: justify;"><em>BY:</em>&nbsp;You must give <a id="appropriate_credit_popup" class="helpLink" href="https://creativecommons.org/licenses/by-sa/4.0/#">appropriate credit</a>, provide a link to the license, and&nbsp;<a id="indicate_changes_popup" class="helpLink" href="https://creativecommons.org/licenses/by-sa/4.0/#">indicate if changes were made</a>. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.</li> <li class="show" style="text-align: justify;"><em>SA:</em>&nbsp; If you remix, transform, or build upon the material, you must distribute your contributions under the <a id="same_license_popup" class="helpLink" href="https://creativecommons.org/licenses/by-sa/4.0/#">same license</a> as the original.</li> </ul> In-vitro antibacterial activity of the seed extract of three-member Artocarpus towards Methicillin-Resistant Staphylococcus aureus (MRSA) https://www.teknolabjournal.com/index.php/Jtl/article/view/237 <p style="text-align: justify;">Methicillin-Resistant <em>Staphylococcus aureus</em> (MRSA) infections have created a critical need for the development of natural antibacterials from a biological source. This research aimed to investigate the antibacterial activity of the seed extract of three-member <em>Artocarpus</em> (<em>Artocarpus heterophyllus</em>, <em>A. champeden</em>, and <em>A. camansi</em>) against MRSA which are the most prevalent causes of infections in patients. Crude seed extracts of three-member <em>Artocarpus</em> were evaluated for their antibacterial activity against MRSA. The antibacterial activity against MRSA of the three extracts was assayed in vitro by the agar well diffusion assay and agar microdilution method and minimum bactericidal concentration. The antibacterial activity, calculated as a zone of inhibition and MIC, MBC values. The Crude seed extracts of three-member <em>Artocarpus</em> showed antibacterial activity against the MRSA in the agar well diffusion assay (1.5-9 mm inhibition diameter). The MIC value of extract showed at 15.62 mg/mL and the MBC value of seed extract of <em>A. heterophyllus</em> at 62.5 mg/mL, <em>A. champeden</em> at 31.25 mg/mL, <em>A. camansi</em> at 250 mg/mL. All seed extracts have the potential to be developed as antibacterial agents, particularly against MRSA strain. Studies on the antibacterial activity against MRSA can provide new information about the benefits seed of members of <em>Artocarpus</em> as a source of natural antibacterial.</p> Muhammad Evy Prastiyanto Inas Hasna Azizah Hafizha Dara Haqi Bagus Dwi Yulianto Aulia Bella Agmala Zhafira Dias Radipasari Nita Ayu Dwi Astuti Arifani Rahma Putri ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2020-12-31 2020-12-31 9 2 128 135 10.29238/teknolabjournal.v9i2.237 Identification of Salmonella typhi contamination by amplification fliC gene in grass-jelly from traditional markets and minimarket in Semarang city https://www.teknolabjournal.com/index.php/Jtl/article/view/207 <p style="text-align: justify;">Grass-jelly is one of the most popular plants consumed by people in various forms. Contamination can cause various diseases, one of those is typhoid fever by <em>Salmonella typhi</em>. The purpose of this study was to detect <em>S. typhi</em> in grass-jelly based on molecular detection by amplification of the <em>fliC</em> gene using PCR. Validation was done by culture methods on SSA media and biochemical testing. The <em>fliC</em> gene amplification results in grass-jelly samples (A1, A2, B1, B2, C1, and C3) showed the DNA fragments size of about 1500 bp. Colony and biochemical characters isolate Peterongan were lead to <em>S. typhi</em>, whereas another isolate was another <em>Salmonella</em> spp. Grass-jelly samples from the Peterongan market in Semarang were positively contaminated by <em>S. typhi </em>and isolate from Pedurungan and the minimarket was another <em>Salmonella</em> spp. Molecular-based food testing is fast enough and accurate for detecting types of bacterial contaminants but the amplification of only the <em>fliC</em> gene cannot specific for <em>S. typhi</em>.</p> Aditya Rahman Ernanto Junita Rensa Palimbongan Anjar Richardo Manufandu Sri Darmawati ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2020-12-31 2020-12-31 9 2 136 144 10.29238/teknolabjournal.v9i2.207 The potential of ethanol extract of white pomegranate leaves (Punica granatum L) as anti-bacterial https://www.teknolabjournal.com/index.php/Jtl/article/view/258 <p style="text-align: justify;">Treatment of infections using penicillin-derived antibiotics such as methicillin has been found to cause antibiotic-resistant bacteria. This bacteria could produce a beta-lactamase enzyme to form a resistant strain. Research on antibacterial activity continues to develop. Pomegranate (Punica granatum L.) was one of the herbal plants whose fruit has long been used for the treatment and prevention of various diseases. This study aimed to determine the potential inhibition of white pomegranate leaf extracts (Punica granatum L.) on the growth of Gram-negative bacteria including Escherichia coli Extended-Spectrum Beta-Lactamase (ESBL) strain and Gram-positive bacteria Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus (MRSA) strain. White pomegranate leaf extract macerated with ethanol 96%, evaporated to obtain pure extracts made with a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and with 100% tested with invitro diffusion method. It was found that the extract of white pomegranate leaves with 30% (10.00 ± 0.0) concentration was able to inhibit the growth of positive Gram bacteria strains MRSA&nbsp; and the extract was unable to inhibit the growth of Escherichia coli bacteria strain ESBL.</p> <p style="text-align: justify;">&nbsp;</p> Ni Made Susilawati I Gede Putu Arnawa Karol Octrisdey Norma Tiku Kambuno ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2020-12-31 2020-12-31 9 2 145 150 10.29238/teknolabjournal.v9i2.258 Erythrocyte index of residents exposed to lead in Tambaklorok, Semarang, Indonesia https://www.teknolabjournal.com/index.php/Jtl/article/view/213 <p style="text-align: justify;">Erythrocyte index can form erythrocyte morphology in the peripheral blood smear preparation. Lead exposure in Tambaklorok exceeds the threshold that affects anemia. The purpose of this study was to determine the erythrocyte index confirmation with erythrocyte morphology in the peripheral blood smear preparation. A cross-sectional study was conducted in 2month in Tambaklorok Semarang residents and 104 samples were taken using the purposive technique. Erythrocyte index was measured using the hematological analyzer and erythrocyte morphology in the peripheral blood smear preparation using Giemsa painting. Erythrocyte index confirmation with the peripheral blood smear preparation was analyzed using the Gamma statistical relationship test. The results showed that the erythrocyte index value was mostly in the normal category, i.e., MCH 68 (64.4%), MCHC 61 (58.6%) and MCH 58 (56%) and below normal category were MCH 45 (42.95%), MCHC 41 (39.4%), and MCV 36 (34.3%). Erythrocyte morphology was mostly hypochromic, namely 46 (44.23%), normochrome 40 (38.46%), and hyperchrome 18 (17.3%). Relationship of MCH with erythrocyte color p-value 0.037 with size p-value 0.038. Conclusion of erythrocyte index confirmation, especially MCH with the peripheral blood smear preparation, there was a match on the color and size of erythrocytes, while the MCV and MCHC values had no significant relationship.</p> Budi Santosa Herlisa Anggraini Ika Dyah Mawarni Niza Muliatun Ardiansyah Nugroho Uly Syarah Siti Atika Risqoeni ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2020-12-31 2020-12-31 9 2 151 158 10.29238/teknolabjournal.v9i2.213 Peripheral stem cell mobilization strategies in patients with autologous hematopoietic cell transplantation: A single center's experience https://www.teknolabjournal.com/index.php/Jtl/article/view/247 <p style="text-align: justify;">This research is to investigate the parameters which may affect the mobilization of stem cells in patients receiving autologous hematopoietic peripheral blood stem cell transplantation (PBSCT). A retrospective study was carried out using the data derived from the medical files of 242 patients who received PBSCT. Descriptive, clinical, and laboratory parameters were compared between patients with successful and unsuccessful stem cell mobilization. Successful stem cell mobilization ratio was 4.463 times higher when preemptive plerixafor was administrated; 1.032 times higher when CD34+ cell count increased 1 unit at the beginning of mobilization. The white blood cell count was inversely correlated with the success of mobilization. An increase of 1 unit in WBC count was associated with a 1.027 times decrease in the success rate. The data indicated that the administration of preemptive plerixafor and CD34+ cell count at the beginning of mobilization were directly related to the success of mobilization after PBSCT. On contrary, WBC count was inversely associated with the success rate.</p> SİNEM NAMDAROĞLU Tugçe Nur Yigenoglu Hikmettullah Batgi Bahar Uncu Ulu Dicle İskender Merih Çakar Mehmet Sinan Dal Fevzi Altuntaş ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2020-12-31 2020-12-31 9 2 159 167 10.29238/teknolabjournal.v9i2.247 Detection of B1 gene as Toxoplasmosis marker in women of childbearing age in West Bandung Regency, Indonesia https://www.teknolabjournal.com/index.php/Jtl/article/view/204 <p style="text-align: justify;">Congenital toxoplasmosis can cause damage and death to the fetus, to prevent this case, toxoplasmosis testing is important for the woman of childbearing age. One of the methods to screening the presence of <em>T. gondii</em> in the blood is Polymerase Chain Reaction (PCR). One of the <em>T. gondii</em> genes which can be used as a marker is the B1 gene. There are many toxoplasmosis cases in Indonesia, but the data is still difficult to find in West Bandung Regency. This study aimed to determine the number of toxoplasmosis cases in a woman of childbearing age in West Bandung Regency using the B1 gene as a marker and to determine the factors that influence these cases by conducting statistical analysis on the results of the questionnaire. The sample used in this study was 50 women of childbearing age (got married and domiciled in West Bandung). All samples have met the inclusion criteria and signed the informed consent. DNA from blood specimens was isolated using the Wizard Genomic DNA Purification Kit. The concentration and purity of isolated DNA were measured using a nanodrop device. Besides, the B1 gene from <em>T. gondii</em> was amplified using a pair of specific primers and visualized by the agarose electrophoresis method. Data were analyzed using the logistic regression method. The results showed that 7 women of childbearing age women (14%) in West Bandung Regency had toxoplasmosis. Frequent contact with pets, especially cats, was a significant factor (p &lt;0.005) in this disease transmission.</p> Patricia Gita Naully Selvi Anggraeni Supendi ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2020-12-31 2020-12-31 9 2 168 175 10.29238/teknolabjournal.v9i2.204 Anti-inflammatory activities of squalene compound of methanol extract of Abroma augusta L https://www.teknolabjournal.com/index.php/Jtl/article/view/228 <p style="text-align: justify;"><em>Abroma augusta</em> L plant traditionally was used to treat swellings, cuts, sores, and bruises. In the province of Jambi, <em>A. augusta</em> is used in folk medicine to treat wounds. This study aims to isolate the steroid compound from the root of <em>A. augusta</em> L and determine its anti-inflammatory activities. Extraction and fractionation have been done with graded maceration using solvents with different polarities, which are n-hexane, ethyl acetate, and methanol. The separation was performed by column chromatography, followed by preparative thin-layer chromatography. The characterization of the isolate was carried out using UV-Vis spectrophotometry and infrared spectrophotometry, GC-MS. The anti-inflammatory activities of methanol extract and isolate of <em>A. augusta</em> was performed in this study was designed to evaluate the dose-response relationship of the anti-inflammatory activity in rat models of chronic inflammation chromatography to obtain isolate 2.1.1 that characterize and showed maximum absorbance at 265. The result of IR showed the presence of functional groups, -C=C-H, -C=H, -CH, CH<sub>3</sub>, CH<sub>2</sub>, and –CO belongs to the steroid compound. The results of the GC-MS show that isolates contain squalene compounds with a value of m/z 410, Isolate and crude extract showed an anti-inflammatory activity that almost approached the positive control of sodium 4-chlorophenolate. It could be concluded that isolate and extract provide good anti-inflammatory activity, that promise for new drug candidate squalene-based <em>A. augusta</em>.</p> Madyawati Latief Muhaimin Muhaimin Hilda Amanda Graha Prahandika Indra Lasmana Tarigan ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2020-12-31 2020-12-31 9 2 176 185 10.29238/teknolabjournal.v9i2.228 Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia https://www.teknolabjournal.com/index.php/Jtl/article/view/235 <p style="text-align: justify;">T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.</p> Miftahul Mushlih Fitri Kumala Sari Hindah Sabrina Amin Siti Asriani Iknan ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2020-12-31 2020-12-31 9 2 186 191 10.29238/teknolabjournal.v9i2.235 The effect of temperature and storage time of cuccal swabs on FGA and D13S317 loci with the STR PCR method https://www.teknolabjournal.com/index.php/Jtl/article/view/243 <p style="text-align: justify;">The samples used for forensic DNA analysis in living individuals are usually blood and buccal swabs, however, blood collection requires an invasive method that can cause discomfort, thus a buccal swab can be a good choice for individuals examined, especially children. This study aimed to determine the effect of temperature and storage time of buccal swabs on the quantity of DNA as material for DNA examination in the forensic field. This study was a laboratory experiment to determine the effect after treatment. Buccal swab samples were 48 and divided into 2 temperature groups, namely room temperature (RT) and 4℃. The division of the temperature groups was also observed with time differences, namely 1, 3, 5, 7 days. EDNA extraction used the DNAzol method and DNA quantification used a Spectrophotometer. The PCR process was carried out with STR primers FGA and D13S317 loci. The visualization stage used acrylamide gel and silver staining.</p> <p style="text-align: justify;">The results of this study prove that there is an effect of temperature and storage time of buccal swab samples. The longer the treatment time, the lower the DNA level. With statistical analysis, it is obtained p-value of &lt;0.005, it can be concluded that there are significant differences in DNA levels at the temperature and storage time treatments of the buccal swab sample. The results of DNA visualization at the FGA and D13S317 loci using the STR PCR method in this study can still be detected and can be used as a reference for examination in forensic cases.</p> <p style="text-align: justify;">&nbsp;</p> Indah Nuraini Masjkur Evy Arfianti Ahmad Yudianto Abdul Hadi Furqoni Qurrota A’yun ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2020-12-31 2020-12-31 9 2 192 198 10.29238/teknolabjournal.v9i2.243