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Decreasing alpha-synuclein aggregation on rotenone-induced adult Zebrafish as Parkinson’s Diseases model by ethanol extraction of Keluwih (Artocarpus camansi) leaves

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Pages 53-60


The prevalence of Parkinson's disease is increasing every year. This progressive disease is characterized by the loss of neurons in the substantia nigra due to the presence of alpha-synuclein aggregates. Keluwih leaves (Artocarpus camansi) are known to have activity in inhibiting acetylcholinesterase, as well as being an antioxidant and anti-inflammatory. The aim of this study was to evaluate the effect of ethanol extract of A. camansi leaves on the levels of alpha-synuclein in male and female adult zebrafish induced with rotenone. The zebrafish were induced with rotenone at a concentration of 5 µg/L for 28 days, along with the administration of 96% ethanol extract of A. camansi leaves at doses of 2.5, 5, 7.5, or 10 mg/L. The media was changed every 48 hours to maintain the concentration of rotenone and extract. After 28 days, alpha-synuclein levels were examined using immunohistochemistry. The administration of ethanol extract of A. camansi leaves can reduce the average levels of alpha-synuclein in male and female adult zebrafish, with the optimum dose being 2.5 mg/L. Therefore, it can be concluded that the administration of ethanol extract of A. camansi leaves can be used as an alternative treatment for Parkinson's disease.

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How to Cite This

Gondokesumo, M. E., Muslikh, F. A., Nopitasari, N. P. D., & Putri, P. D. A. (2023). Decreasing alpha-synuclein aggregation on rotenone-induced adult Zebrafish as Parkinson’s Diseases model by ethanol extraction of Keluwih (Artocarpus camansi) leaves. Jurnal Teknologi Laboratorium, 12(2), 53–60. https://doi.org/10.29238/teknolabjournal.v12i2.408

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