Potensi Antibakteri Isolat Actinomycetes terhadap Aktivitas Proteolitik dan Amilolitik Escherichia Coli ATTC 25922
Occurs E. coli resistance to class 3 cephalosporin class antibiotics and fluoroquinolone groups. The antibiotic resistance that occurs has narrowed the choice of therapy. This study aims to determine the effect of Actinomycetes isolates on proteolytic and amylolytic enzymes E. coli ATCC 25922. This research is experimental research, qualitative tests of protease and amylase enzymes from E. coli ATCC 25922 shown by clear zones around the growing colonies. The result of ANOVA One-Way test showed a significant difference in the width of clear zone, colony zone and PER and AER score with p-value < 0,05. This indicates that Actinomycetes isolates contain compounds that can act as inhibitors of protease and amylase enzymes from E.coli ATCC 25922. It is hoped that there will be research about the identification of Actinomycetes species isolates in Bogor Botanical Garden so that later can be cultivated and produced as an antibiotics alternative.
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2. Brooks GF, Butel JS, Morse SA, Jawetz, Melnick, Adelberg. Mikrobiologi Kedokteran.
Jakarta: Penerbit Buku Kedokteran EGC; 2008.
3. Lavor AKLS, Et.al. Association between drugs and herbal products: in vitro enhancement of
the antibiotic activity by fractions from leaves of Croton campestris A. (Euphorbiaceae). Eur
J Integr Med. 2014;6.
4. Martha D, Achmad S, Tejasari M. Escherichia coli resisten terhadap seftriakson dan
siprofloksasin. In: Pendidikan Dokter Universitas Islam Bandung. Bandung; 2015.
5. Adriani Y, Febriwanti T. Isolasi dan Karakterisasi Actinomycetes Sebagai Penghasil
Antibiotik Dari Sampel Peternakan Sapi di Kecamatan Galesong Kabupaten Takala.
6. Nediakolva D, Naidenova M. Screening The Antimicrobial Activity of Actinomycetes Strains
Isolated from Antartica. J Cult Collect. 2005;4:29-35.
7. Pandhare J. tudies of an alkaline protease inhibitor from a Streptomyces sp. Div Biochem
Sci Natl Chem Lab. 2002.
8. Karthik L, Et.al. Protease Inhibitors From Marine Actinobacteria as a Potential Source for
Antimalarial Compound. PLoS One. 2014;9(3). doi:10.1371/journal.pone.0090972.
9. Raja S, Ganesan S, Sivakumar K, Thangaradjou T. Screening of marine actinobacteria for
amylase enzymes inhibitors. Indian J Microbiol. 2010;50:233.
10. Meng P, Xie C, Geng P, Et.al. Inhibitory effect of componentsfrom Streptomyces species on
a-glucosidase and a-amylase of different origin. Appl Biochem Microbiol. 2003;49(8):160.
11. Zhibin S, Et.al. Isolation and characterization of a Proteinaceous Î±-amylase inhibitor AAICC5
from Streptomyces sp. CC5 and Itâ€™s Gene Cloning and Expression. PubMed. 2014.
12. Nurhalijah S. Isolasi Bakteri Dan Uji Aktivitas Amilase Kasar. Medan; 2008.
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