Efektivitas Variasi Garam Salmiak (NH4Cl) Dan Sentrifugasi Pada Pemeriksaan Basil Tahan Asam Penderita Tuberculosis

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Saadah Siregar Yati Supriatin Lanny Noor

Abstract

According to a report by WHO Indonesia, it is a case number three infected with tuberculosis. Tuberculosis disease is caused by Mycobacterium tuberculosis that belongs to acid-fast bacilli bacteria (BTA). Sputum smear examination microscopically still has many deficiencies caused by mucoprotein in sputum so that BTA cannot be separated from sputum, this is causing a false negative in reading result Microscope. The objective of this research is to know the most effective variation of concentration, speed and time of centrifugation on smear examination in tuberculosis patients. The research method used is experimental method and Design of complete Random Block design. Data were analyzed by statistic test with ANOVA test on SPSS. The result of the research is that at concentration 2% NH4Cl It can increase the number of BTA with optimal centrifugation speed / effective at 5000 rpm for 15 minutes. Suggestion for further research is to use salmaniac salt concentration with a concentration lower than 2%.

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How to Cite
SIREGAR, Saadah; SUPRIATIN, Yati; NOOR, Lanny. Efektivitas Variasi Garam Salmiak (NH4Cl) Dan Sentrifugasi Pada Pemeriksaan Basil Tahan Asam Penderita Tuberculosis. Jurnal Teknologi Laboratorium, [S.l.], v. 6, n. 2, p. 46 - 55, sep. 2017. ISSN 2580-0191. Available at: <http://www.teknolabjournal.com/index.php/Jtl/article/view/91>. Date accessed: 14 dec. 2017. doi: https://doi.org/10.29238/teknolabjournal.v6i2.91.
Section
Bacteriology

References

[1]. Widoyono M. Penyakit Tropis Epidemiologi Penularan, Pencegahan dan Pemberantasan. Edisi II. Penerbit Erlangga.Jakarta.2001

[2]. Wahyuningsih E. Pola Klinik Tuberkulosis Paru di RSUP Dr. Kariadi Semarang Periode Juli 2012-Agustus 2013: Faculty of Medicine Diponegoro University; 2014.

[3]. Brooks GF, Butel JS, Morse SA. Medical microbiology. United States 25th. 2006.

[4]. Chen P, Shi M, Feng G-D, Liu J-Y, Wang B-J, Shi X-D, et al. A highly efficient Ziehl-Neelsen stain: identifying de novo intracellular Mycobacterium tuberculosis and improving detection of extracellular M. tuberculosis in cerebrospinal fluid. Journal of clinical microbiology. 2012;50(4):1166-70.

[5]. Yamada H, Mitarai S, Aguiman L, Matsumoto H, Fujiki A. Preparation of Mycobacteria-containing artificial sputum for TB panel testing and microscopy of sputum smears. The International Journal of Tuberculosis and Lung Disease. 2006;10(8):899-905.

[6]. Bambang Supriyantono SP, Muji Rahayu. Amonium klorida 10% dan Sentrifugasi pada penemuan bakteri Mycobacterium tuberculosistuberculosisuntuk diagnosis Tuberculosis Paru. Jurnal Teknologi Kesehatan. 2011;7 No. 2.

[7]. Merryani girsang s, Dany R, Tami, Irawati olli, Gendero Wahyuno. Teknik sentrifugasi untuk meningkatkan penemuan bakteri tahan asam dari sputum TB metode Ziehl Neelsen. J Badan Litbang Depkes. 2003;13(4):23-1

[8]. Tjay TH, Rahardja K. Obat-obat penting: khasiat, penggunaan dan efek-efek sampingnya: Elex Media Komputindo; 2007

[9]. Said M. Pengolahan Air Limbah Laboratorium dengan Menggunakan Koagulan Alum Sulfat dan Poli Aluminium Klorida (PAC)”. Jurnal Penelitian Sains. 2009;9:12-8.

[10]. Sacher RA, McPherson RA, editors. Tinjauan Klinis Hasil Pemeriksaan, Laboratorium 2004: EGC

[11]. Sarengat N, Yuniari A, Setyorini I, Suyatini S, editors. Kajian Potensi Pencemaran Industri pada Lingkungan Perairan Didaerah Istimewa Yogyakarta. Prosiding Seminar Nasional Kulit, karet dan Plastik; 2015

[12]. Soekidjo N. Metodologi penelitian kesehatan. Jakarta: Rineka Cipta. 2010:50-5.

[13]. Jasaputra DK, Widjaja JT, Wargasetia TL, Makangiras I. Deteksi Mycobacterium tuberculosis dengan Teknik PCR pada Cairan Efusi Pleura Penderita Tuberkulosis Paru. Jurnal Kedokteran Maranatha. 2010;7(1):pp. 86-90

[14]. Sayekti E, Imelda H, Titin A. Reaksi Substitusi Gugus Hidroksi Pada Sitronelol dengan Klorida Menggunakan Campuran Amonium Klorida dan Asam Sulfat. ALCHEMY jurnal penelitian kimia. 2015;11(2):135-46