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Examination of glucose levels of the GOD-PAP method in serum may be disrupted by the presence of turbidity caused by lipemic serum, thus causing high false serum glucose levels to result. The addition of alpha-cyclodextrin (α-CD) may bind the lipemic in serum. This study aims to find out how the concentration and time of optimal centrifugation with the addition of alpha-cyclodextrin. Added variation alpha-cyclodextrin concentration 0.5%, 1%, and 1.5% in pooled serum with variation concentration of triglycerides ± 1000 mg/dL, ± 1500 mg/dL and ± 2000 mg/dL incubated for 30 minute at 4°C. Centrifuged at 3000 rpm with variation of centrifugation time for 5 minutes, 10 minutes and 15 minutes at 4°C to form precipitate and supernatant. Then the supernatant measured the glucose GOD-PAP and triglycerides GPO-PAP using a photometer. The result were no significant differences in variations centrifugation time and variations alpha-cyclodextrin concentrations after comparison with serum glucose pooled levels (base line) in the ANOVA test, Kruskall Wallis Test and Mann Whitney test. There was no significant difference with pooled serum (base line) at the time of triglyceride concentration ± 1000 mg/dL with alpha-cyclodextrin concentration 0.5% and centrifugation time at 10 minute, triglyceride concentration ± 1500 mg/dL and ± 2000 mg/dL with alpha-cyclodextrin concentration 1%, centrifugation time at 5 minute.
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2. Hoon SD, Juwon K, Young U, et al. No TitlDevelopment of an Integrated Reporting System for Verifying Hemolysis , Icterus , and Lipemia in Clinical Chemistry Results e. Ann Lab Med J. 2014;34(4):307.
3. Nora N. Lipemia : causes, interference mechanisms, detection and managemente. Biochem Medica J. 2014;24(1):57-67.
4. Goce D. Interference Testing. Clin Biochem Rev J. 2008;29(8):43-48.
5. Sandhya M, R DS, D KM. Frequency and causes of lipemia interference of clinical chemistry laboratory tests. Elsevier. 2017;8(2):1-9.
6. S YD. Effect of Drugs on Clinical Laboratory Tests , 1995 dalam Biolabo., “Glucose GOD-PAP,” Maizy, 2011. AACC Press. 1995;4:274-294.
7. Ajit S, Karen A, W BJ. Flocculation of serum lipoproteins with cyclodextrins: Application to assay of hyperlipidemic serum. Clin Chem J. 1990;36(3):529-532.
8. Radišić BV, Sandra B, Maja K, Andrea R, M LV. Serum delipidation but not high-speed centrifugation is effective in clearing lipemia interference in serum lipase activity measurement. Clin Chem Lab Med J. 2016;54:247–249.
9. Jianxiang Z, X MP. Cyclodextrin-based supramolecular systems for drug delivery: Recent progress and future perspective,". Adv Drug Deliv Rev J. 2014;65(9):1–39.
10. Amran L. Produksi Siklodekstrin dari Substrat Tapioka dengan Menggunakan Pullulanase dan CGTase secara Simultan. J Tek Ind Pert. 2011;18(2):99-105.
11. Crestani de MJ, Azevedo MTE, Francisco V, Gomes FH. Cyclodextrins and ternary complexes: Technology to improve solubility of poorly soluble drugs. Brazilian J Pharm Sci. 2011;47(4):665-681.
12. Neil FE. The Effect Of Alpha-Cyclodextrin On Acute Blood Lipid And Glycemic Responses To A Fat Containing Meal. Wayne State Univ. 2013.
13. Fajar PA, Tri WS, Budi S. Serum Lipemik Dengan Flokulan Gamma-Siklodekstrin Pada Pemeriksaan Glukosa. Med Lab Technol J. 2017;3(2):68-72.
14. Sunheimer RL, Threatte GA, Pincus MR, Lifshitz MS. Analysis: Principle of Instrumentation. In: McPherson RA, Pincus MR, eds. HENRY’S Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Philadelphia: Elsevier Saunders; 2011:47.
15. Nadya BA. Penggunaan Siklodekstrin Dalam Bidang Farmasi. Maj Farm. 2014;10(1):197-201.