Main Article Content
Occurs E. coli resistance to class 3 cephalosporin class antibiotics and fluoroquinolone groups. The antibiotic resistance that occurs has narrowed the choice of therapy. This study aims to determine the effect of Actinomycetes isolates on proteolytic and amylolytic enzymes E. coli ATCC 25922. This research is experimental research, qualitative tests of protease and amylase enzymes from E. coli ATCC 25922 shown by clear zones around the growing colonies. The result of ANOVA One-Way test showed a significant difference in the width of clear zone, colony zone and PER and AER score with p-value < 0,05. This indicates that Actinomycetes isolates contain compounds that can act as inhibitors of protease and amylase enzymes from E.coli ATCC 25922. It is hoped that there will be research about the identification of Actinomycetes species isolates in Bogor Botanical Garden so that later can be cultivated and produced as an antibiotics alternative.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Publishing your paper with Jurnal Teknologi Laboratorium (JTL) means that the author or authors retain the copyright in the paper. JTL granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by JTL in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.
JTL journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially.
JTL journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:
- BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- SA: If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
. Arisman., "Buku Ajar Ilmu Gizi Keracunan Makanan," Penerbit Buku Kedokteran EGC, Jakarta, 2009.
. Lavor, AKLS, et.al., "Association between drugs and herbal products: in vitro enhancement of the antibiotic activity by fractions from leaves of Croton campestris A. (Euphorbiaceae)’, European Journal of Integrative Medicine, Vol.6, Maret 2014.
. Martha, D, Achmad, S, Tejasari., M., "Escherichia coli resisten terhadap seftriakson dan siprofloksasin" : Prosiding Pendidikan Dokter Universitas Islam Bandung, Bandung, 2015.
. Adriani, Y & Febriwanti, T., "Isolasi dan Karakterisasi Actinomycetes Sebagai Penghasil Antibiotik Dari Sampel Peternakan Sapi di Kecamatan Galesong Kabupaten Takalar, " Biogenesis, vol.1, no.2, Desember, hlm.97-100, 2013.
. Nediakolva, D & Naidenova,M., "Screening The Antimicrobial Activity of Actinomycetes Strains Isolated from Antartica," Journal of Culture Collection Vol.4, 2004-2005, pp.29-35. 2005.
. Ambarwati dan Azizah Gama T., "Isolasi Actinomycetes Dari Tanah Sawah Sebagai Penghasil Antibiotik," Jurnal Penelitian Sains & Teknologi, Vol. 10, No. 2, 2009: 101 – 111.
. Pandhare, Jui., "Studies of an alkaline protease inhibitor from a Streptomyces sp.," Division of Biochemical Sciences National Chemical Laboratory. Pune 411 008 India June 2002.
. Karthik L, et.al., "Protease Inhibitors From Marine Actinobacteria as a Potential Source for Antimalarial Compound," Published online 2014 Mar.doi : 10.1371/journal.pone.0090972. PLoS One.; 9(3): e90972, 2014.
. Raja S, Ganesan S, Sivakumar K, Thangaradjou T. "Screening of marine actinobacteria for amylase enzymes inhibitors," Indian J Microbiol 50:233–7. 2010.
. Meng P, Xie C, Geng P, et al., "Inhibitory effect of componentsfrom Streptomyces species on a-glucosidase and a-amylase of different origin, " Appl Biochem Microbiol 49:160–8, 2013.
. Sun Zhibin, et.al., "Isolation and characterization of a Proteinaceous α-amylase inhibitor AAI-CC5 from Streptomyces sp. CC5 and It’s Gene Cloning and Expression," Article in Antonie van Leeuwenhoek • November 2014 DOI: 10.1007/s10482-014-0333-y • Source: PubMed. Received: 16 September 2014 / Accepted: 11 November 2014 / Published online: 20 November 2014.
. Sutiamiharja Nurhalijah, "Isolasi Bakteri Dan Uji Aktivitas Amilase Kasar," Sekolah Pascasarjana Universitas Sumatera Utara. Medan, 2008.